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hs27 human normal fibroblast cell line (ATCC)

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ATCC hs27 human normal fibroblast cell line
Hs27 Human Normal Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hs27 human normal fibroblast cell line/product/ATCC
Average 96 stars, based on 657 article reviews

hs27 human normal fibroblast cell line - by Bioz Stars, 2026-05

96/100 stars

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Effect of treatment for 24–48–72 h with Buxus sempervirens hydroalcoholic extract (BSHE) on cell proliferation and viability as determined by the trypan blue exclusion test (A) and the MTS assay (B) . HS27: normal human dermal fibroblasts; HCT116: human colorectal carcinoma cells; BMel: human melanoma cells; PC3: human prostate cancer cells. Graphs on the left columns of both panels show the effect of various BSHE concentrations ( x -axis, logarithmic values) on normalized growth rate inhibition (GR, see Materials and Methods) as reported on y -axis. Graphs on the right columns of both panels show the effect of various BSHE concentrations ( x -axis, logarithmic values) on relative cell viability ( y -axis), i.e., number of viable cells (or net A490 nm using MTS assay) under BSHE treatment versus BSHE-untreated controls. BSHE concentrations were 3.9, 7.8, 15.6, 31.2, 62.5, 125, 250, and 500 μg/mL. Means ± S.D. were reported (n = 3 for the trypan blue exclusion test, n = 4 for the MTS assay). Graphs were obtained from the online tool GR calculator ( www.grcalculator.org ; see Materials and Methods).

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Effect of treatment for 24–48–72 h with Buxus sempervirens hydroalcoholic extract (BSHE) on cell proliferation and viability as determined by the trypan blue exclusion test (A) and the MTS assay (B) . HS27: normal human dermal fibroblasts; HCT116: human colorectal carcinoma cells; BMel: human melanoma cells; PC3: human prostate cancer cells. Graphs on the left columns of both panels show the effect of various BSHE concentrations ( x -axis, logarithmic values) on normalized growth rate inhibition (GR, see Materials and Methods) as reported on y -axis. Graphs on the right columns of both panels show the effect of various BSHE concentrations ( x -axis, logarithmic values) on relative cell viability ( y -axis), i.e., number of viable cells (or net A490 nm using MTS assay) under BSHE treatment versus BSHE-untreated controls. BSHE concentrations were 3.9, 7.8, 15.6, 31.2, 62.5, 125, 250, and 500 μg/mL. Means ± S.D. were reported (n = 3 for the trypan blue exclusion test, n = 4 for the MTS assay). Graphs were obtained from the online tool GR calculator ( www.grcalculator.org ; see Materials and Methods).

Article Snippet: The Hs27 normal human dermal fibroblast cell line (ATCC 1634-CRL), the HCT116 human colorectal carcinoma cell line (ATCC CCL-247) and the PC3 human prostate cancer cell line (ATCC 11435-CRL) were purchased by American Type Culture Collection (Manassas, Va), while the BMel human melanoma cell line was a gift from Prof. P.G.Natali (“Regina Elena” Institute for Cancer Research, Rome, Italy).

Techniques: MTS Assay, Inhibition

GR50 and IC50 values (µg/mL) of Buxus sempervirens hydroalcoholic extract in HS27 normal human dermal fibroblasts, HCT116 human colorectal carcinoma cells, BMel human melanoma cells and PC3 human prostate cancer cells at different exposure times.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: GR50 and IC50 values (µg/mL) of Buxus sempervirens hydroalcoholic extract in HS27 normal human dermal fibroblasts, HCT116 human colorectal carcinoma cells, BMel human melanoma cells and PC3 human prostate cancer cells at different exposure times.

Techniques:

Representative inverted phase-contrast microscopy pictures of Buxus sempervirens hydroalcoholic extract (BSHE)-untreated cells (A,C,E and G) and cells treated (B,D,F and H) for 48 h with BSHE at GR 50 concentrations of 72, 48, 38, and 32 μg/mL for HS27, HCT 116, PC3 and BMel cells, respectively). a,b: HS27 normal human skin fibroblasts; c,d: HCT 116 colorectal carcinoma cells; e,f: BMel melanoma cells; g,h: PC3 prostate cancer cells. BSHE-treated cells show vesicle accumulation in the cytoplasm mainly around nuclei. Scale bar, 40 μm.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Representative inverted phase-contrast microscopy pictures of Buxus sempervirens hydroalcoholic extract (BSHE)-untreated cells (A,C,E and G) and cells treated (B,D,F and H) for 48 h with BSHE at GR 50 concentrations of 72, 48, 38, and 32 μg/mL for HS27, HCT 116, PC3 and BMel cells, respectively). a,b: HS27 normal human skin fibroblasts; c,d: HCT 116 colorectal carcinoma cells; e,f: BMel melanoma cells; g,h: PC3 prostate cancer cells. BSHE-treated cells show vesicle accumulation in the cytoplasm mainly around nuclei. Scale bar, 40 μm.

Techniques: Microscopy

Representative fluorescence microscopy pictures of Buxus sempervirens hydroalcoholic extract (BSHE)-untreated cells (A,C,E and G) and cells treated (B,D,F and H) for 48 h with BSHE at GR 50 concentrations of 72, 48, 38 and 32 μg/mL for HS27, HCT 116, PC3 and BMel cells, respectively. Cells were stained with acridine orange (AO) and images were acquired using a color camera. In live cells, AO is accumulated by acidic vesicles yielding strong orange or red signals. BSHE-treated cells show a great increase of these vesicles. Scale bar, 40 μm.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Representative fluorescence microscopy pictures of Buxus sempervirens hydroalcoholic extract (BSHE)-untreated cells (A,C,E and G) and cells treated (B,D,F and H) for 48 h with BSHE at GR 50 concentrations of 72, 48, 38 and 32 μg/mL for HS27, HCT 116, PC3 and BMel cells, respectively. Cells were stained with acridine orange (AO) and images were acquired using a color camera. In live cells, AO is accumulated by acidic vesicles yielding strong orange or red signals. BSHE-treated cells show a great increase of these vesicles. Scale bar, 40 μm.

Techniques: Fluorescence, Microscopy, Staining

Representative indirect immunofluorescence images indicating accumulation of microtubule-associated light chain-3 protein (LC3) in Buxus sempervirens hydroalcoholic extract (BSHE)-untreated cells (A,C,E and G) and cells treated (B,D,F and H) for 48 h with BSHE at GR 50 concentrations of 72, 48, 38, and 32 μg/mL for HS27, HCT 116, PC3 and BMel cells, respectively. Immunofluorescence analysis, carried out by using anti-LC3 antibodies, evidenced cell LC3 puncta accumulation following BSHE treatment. Nuclei are in blue (DAPI). Scale bar, 40 μm.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Representative indirect immunofluorescence images indicating accumulation of microtubule-associated light chain-3 protein (LC3) in Buxus sempervirens hydroalcoholic extract (BSHE)-untreated cells (A,C,E and G) and cells treated (B,D,F and H) for 48 h with BSHE at GR 50 concentrations of 72, 48, 38, and 32 μg/mL for HS27, HCT 116, PC3 and BMel cells, respectively. Immunofluorescence analysis, carried out by using anti-LC3 antibodies, evidenced cell LC3 puncta accumulation following BSHE treatment. Nuclei are in blue (DAPI). Scale bar, 40 μm.

Techniques: Immunofluorescence

Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on autophagic proteins levels in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). (A) : Histograms showing the relative intensity of the western blot bands of LC3II, p62 and beclin in BSHE-untreated (control, ctrl) and–treated cells (trt). Densitometry values were normalized for β-actin and compared with control. (B) : Representative western blots including the LC3-I ones. (C) : Table showing the LC3II/LC3I ratio, as sensitive index of autophagy, in the above BSHE-untreated and-treated cells; abbreviations: ctrl and trt as in (A) . Means ± S.E.M. are reported in A and C (n = 3 or more) where the Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on autophagic proteins levels in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). (A) : Histograms showing the relative intensity of the western blot bands of LC3II, p62 and beclin in BSHE-untreated (control, ctrl) and–treated cells (trt). Densitometry values were normalized for β-actin and compared with control. (B) : Representative western blots including the LC3-I ones. (C) : Table showing the LC3II/LC3I ratio, as sensitive index of autophagy, in the above BSHE-untreated and-treated cells; abbreviations: ctrl and trt as in (A) . Means ± S.E.M. are reported in A and C (n = 3 or more) where the Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01; *** p < 0.001.

Techniques: Western Blot, Control

Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on p21, p27 and p53 levels in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). Histograms showing relative intensity of the western blot bands of these proteins in BSHE-untreated (control, ctrl) and-treated cells (trt) along with representative western blots are shown. Densitometry values were normalized for β-actin and compared with control. Means ± S.E.M. are reported (n = 3 or more). The Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on p21, p27 and p53 levels in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). Histograms showing relative intensity of the western blot bands of these proteins in BSHE-untreated (control, ctrl) and-treated cells (trt) along with representative western blots are shown. Densitometry values were normalized for β-actin and compared with control. Means ± S.E.M. are reported (n = 3 or more). The Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01.

Techniques: Western Blot, Control

Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on cyclins D1, E and B1 in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). Histograms showing relative intensity of the western blot bands of these proteins in BSHE-untreated (control, ctrl) and–treated cells (trt) along with representative western blots are shown. Densitometry values were normalized for β-actin and compared with control. Means ± S.E.M. are reported (n = 3 or more). The Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on cyclins D1, E and B1 in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). Histograms showing relative intensity of the western blot bands of these proteins in BSHE-untreated (control, ctrl) and–treated cells (trt) along with representative western blots are shown. Densitometry values were normalized for β-actin and compared with control. Means ± S.E.M. are reported (n = 3 or more). The Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01.

Techniques: Western Blot, Control

Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on levels of the apoptotic proteins survivin, BAX and Bcl2 in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). Histograms showing relative intensity of the western blot bands of these proteins in BSHE-untreated (control, ctrl) and -treated cells (trt) along with representative western blots are shown. Densitometry values were normalized for β-actin and compared with control. Means ± S.E.M. are reported (n = 3 or more). The Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Hydroalcoholic extract of Buxus sempervirens shows antiproliferative effect on melanoma, colorectal carcinoma and prostate cancer cells by affecting the autophagic flow

doi: 10.3389/fphar.2023.1073338

Figure Lengend Snippet: Effect of Buxus sempervirens hydroalcoholic extract (BSHE) on levels of the apoptotic proteins survivin, BAX and Bcl2 in HS27, HCT116, PC3 and BMel cell lines following their treatment for 24/48 h with the respective GR 50 concentrations (at 48 h) of BSHE (see ). Histograms showing relative intensity of the western blot bands of these proteins in BSHE-untreated (control, ctrl) and -treated cells (trt) along with representative western blots are shown. Densitometry values were normalized for β-actin and compared with control. Means ± S.E.M. are reported (n = 3 or more). The Student’s t-test was employed to compare the values of BSHE-untreated and -treated cells. * p < 0.05; ** p < 0.01; *** p < 0.001.

Techniques: Western Blot, Control

RT-PCR and Immunohistochemistry analyses of expression of TP73 and related-genes in human cell lines and tissues. (A ) A schematic representation of several genes located in chromosome 1p36.32.; ( B) Expression of TP73 gene and related-genes in normal human liver stem cells (HepCY & HepCO), HCC & GI cancer cell lines and normal human skin fibroblasts cell line (HS27): (Panel I) Tumor protein TP73 and PRDM16 , MEGF6 , CEP104 and DFFB expression level close to the TP73 gene located in chromosome 1p36.32 region, (II) related genes; ( C ) Immunohistochemistry analyses of TP73 expression in normal and HCC patients’ Cancer-adjacent tissue and Cancer tissues objective magnification: 10X upper panel (Scale Bar: 100 µm) and 40× lower panel (Scale Bar: 20 µm), three specimens were detected for each. This experiment was repeated three times with three different tissues to confirm the results. Note: The row of bands representing the expression of each gene and separated by white spaces as shown in the gels displayed in B-I and B-II are cropped from full-length gels of the corresponding genes . The same exposures were made for each gel . The original gels for each figure are shown in the Supplementary Information File .

Journal: Scientific Reports

Article Title: DNA Methylation Activates TP73 Expression in Hepatocellular Carcinoma and Gastrointestinal Cancer

doi: 10.1038/s41598-019-55945-7

Figure Lengend Snippet: RT-PCR and Immunohistochemistry analyses of expression of TP73 and related-genes in human cell lines and tissues. (A ) A schematic representation of several genes located in chromosome 1p36.32.; ( B) Expression of TP73 gene and related-genes in normal human liver stem cells (HepCY & HepCO), HCC & GI cancer cell lines and normal human skin fibroblasts cell line (HS27): (Panel I) Tumor protein TP73 and PRDM16 , MEGF6 , CEP104 and DFFB expression level close to the TP73 gene located in chromosome 1p36.32 region, (II) related genes; ( C ) Immunohistochemistry analyses of TP73 expression in normal and HCC patients’ Cancer-adjacent tissue and Cancer tissues objective magnification: 10X upper panel (Scale Bar: 100 µm) and 40× lower panel (Scale Bar: 20 µm), three specimens were detected for each. This experiment was repeated three times with three different tissues to confirm the results. Note: The row of bands representing the expression of each gene and separated by white spaces as shown in the gels displayed in B-I and B-II are cropped from full-length gels of the corresponding genes . The same exposures were made for each gel . The original gels for each figure are shown in the Supplementary Information File .

Article Snippet: HCC (HepG2, SNU3958, SNU449, and SNU475) and GI (Caco2 and HCT116) cancer cell lines, and normal human skin fibroblasts cell line (HS27) were obtained from ATCC (Manassas, VA) and cultured following the instructions of the supplier.

Techniques: Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Expressing

DNA methylation pattern of TP73 gene promoter in normal human liver stem cells, HCC&GI cancer cell lines and normal human skin fibroblasts cell line. ( A) Schematic outline for the sequence of TP73 promoter and CpG islands. (B) Methylation status of TP73 promoter in normal human liver stem cells (HepCY & HepCO), HCC & GI cancer cell lines and normal human skin fibroblasts cell line (HS27) detected by MSPCR (C) DNA methylation pattern of TP73 gene promoter in normal human liver stem cells (HepCY & HepCO), HCC & GI cancer cell lines and normal human skin fibroblasts cell line (HS27) identified by bisulfite sequencing. Note: The row of bands representing the expression of each gene and separated by white spaces as shown in the gels displayed in panel B are cropped from full-length gels of the corresponding genes . The same exposures were made for each gel . The original gels for each figure are shown in the Supplementary Information File .

Journal: Scientific Reports

Article Title: DNA Methylation Activates TP73 Expression in Hepatocellular Carcinoma and Gastrointestinal Cancer

doi: 10.1038/s41598-019-55945-7

Figure Lengend Snippet: DNA methylation pattern of TP73 gene promoter in normal human liver stem cells, HCC&GI cancer cell lines and normal human skin fibroblasts cell line. ( A) Schematic outline for the sequence of TP73 promoter and CpG islands. (B) Methylation status of TP73 promoter in normal human liver stem cells (HepCY & HepCO), HCC & GI cancer cell lines and normal human skin fibroblasts cell line (HS27) detected by MSPCR (C) DNA methylation pattern of TP73 gene promoter in normal human liver stem cells (HepCY & HepCO), HCC & GI cancer cell lines and normal human skin fibroblasts cell line (HS27) identified by bisulfite sequencing. Note: The row of bands representing the expression of each gene and separated by white spaces as shown in the gels displayed in panel B are cropped from full-length gels of the corresponding genes . The same exposures were made for each gel . The original gels for each figure are shown in the Supplementary Information File .

Techniques: DNA Methylation Assay, Sequencing, Methylation, Methylation Sequencing, Expressing

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